Microfabrication by multi-photon polymerization is a direct laser-write technique which allows 3D structuring of photopolymers at the micro- and nano-scale. This can be achieved through combination of various nonlinear effects, careful consideration of laser radiation parameters and precise focusing conditions. For this reason 3D laser lithography was used for creation of functional devices in fields of nanophotonics, microoptics, microfluidics, micromechanics, tissue engineering and much more. It is important to note, that there is huge variety of materials that can be processed by applying 3D laser lithography, including hybrid organic‑inorganic photopolymers, biodegradable polymers, elastomers, proteins and so on.