The use of 3D femtosecond laser printing in bioscience is a relatively new and emerging field that involves the use of 3D printing technology to create highly detailed and complex biological structures using femtosecond lasers. This technology is being used to create a wide range of biological structures, such as cells, tissues, and organs, for use in drug development, tissue engineering, and medical research. The use of femtosecond lasers allows for the creation of highly precise and intricate structures, which can improve the performance of biological structures. Additionally, 3D femtosecond laser printing can be used to produce large quantities of biological structures quickly and efficiently, making it a valuable tool for bioscience researchers and developers.
One of the best ways to produce them is the multi-photon polymerization technique which enables the production of precise and firm microneedles. Even more complex shapes of polymeric needles can be obtained by maintaining the high sharpness of the needle tips.
Tesla valve microfluidic channels can be fabricated inside the volume of glass. This microchannel design allows the liquid to flow in only one direction.
Micro Channels Formation
The SLE technique makes it possible to produce taper-free micron precision channels with a low surface roughness of ~200 nm RMS.
The hybrid-fabrication approach enables rapid production of channels out of fused silica via laser ablation, while multiphoton polymerization is used to integrate fine-mesh 3D filters of arbitrary geometry inside the channel.
The channels in the glass along with polymeric micropillars form a microfluidic device where different types of inserted cells can form a complex cellular architecture and manipulate cell‑to-cell interactions.
By selective laser etching (SLE), glass microstructures can be made and polymeric structures can be integrated into the glass microstructures using multiphoton polymerization (MPP).
Scaffold-like structure can be produced within a resolution of up to 150 nm capable of entrapping submicrometer sized cells by multiphoton polymerization (MPP) technique.
Multi-photon polymerization (MPP) enables the production of precise and strong microlenses and microneedles for visualization, filtering, and drug delivery. MPP can also be combined with other fabrication techniques such as selective laser etching to create hybrid microfabricated systems, like glass and polymer structures.
Femtosecond microfabrication is a hybrid approach that combines laser ablation and multi-photon polymerization for lab-on-chip applications. This enables the rapid production of channels and filters in arbitrary geometries for devices such as a microfluidic macromolecule separator and a liver-on-chip model.
Femtosecond microfabrication technology is applied in microfluidics through 3D laser lithography and selective laser etching (SLE). 3D laser lithography is used to produce micro filters and sensors, while SLE enables the production of complex-shaped microfluidic channels out of fused silica glass with low surface roughness and high precision.
Multi-photon polymerization (MPP) can be used for meta-material fabrication, including scaffolds for tissue engineering. The 3D gyroid structure is a mechanically rigid, light structure that can be fabricated by MPP, with a resolution up to 150 nm and capable of entrapping submicrometer sized cells.
Microfabrication by multi-photon polymerization is a direct laser-write technique which allows 3D structuring of photopolymers at the micro- and nano-scale.
Selective Laser Etching
Selective laser etching (SLE) is a subtractive laser technology allowing fabrication of complex-shape 3D glass parts with micrometer precision.
Multiphoton-polymerization (MPP) is a technology that enables the production of arbitrary shape polymeric structures within submicrometric resolution. First, a photoresist sample is prepared by drop-casting polymer material mixed with a photoinitiator on the glass slide and then pre-baking.
Laser Nanofactory workstation allows hybrid fabrication, meaning that various processes are supported by the same equipment. The two of our most frequently used processes are multiphoton polymerization and selective glass etching, however that is far from all!
Contract Research Services
A feasibility study is composed of several steps, including researching methods for fabricating micro-structures, fabricating a micro-structure prototype, measuring and aligning the prototype with technical requirements, and finally preparing a study report.
Stitchless support-free 3D printing of free-form micromechanical structures with feature size on-demand
L. Jonušauskas, T. Baravykas, D. Andrijec, T. Gadišauskas, and V. Purlys. Sci Rep 9, 17533 (2019). DOI: 10.1038/s41598-019-54024-1.
Optimization of selective laser etching (SLE) for glass micromechanical structure fabrication
A. Butkutė, T. Baravykas, J. Stančikas, T. Tičkūnas, R. Vargalis, D. Paipulas, V. Sirutkaitis, and L. Janušauskas. Optical Express 23487, Vol. 29, No. 15, 19.07.2021, (2021). DOI: 10.1364/OE.430623.
3D Manufacturing of Glass Microstructures Using Femtosecond Laser
A. Butkutė, and L. Jonušauskas. Micromachines 2021, 12, 499, (2021). DOI: 10.3390/mi12050499.
Hybrid additive subtractive femtosecond 3D manufacturing of nanofilter based microfluidic separator
D. Andrijec, D. Andriukaitis, R. Vargalis, T. Baravykas, T. Drevinskas, O. Kornyšova, A. Butkutė, V. Kaškonienė, M. Stankevičius, H. Gricius, A. Jagelavičius, A. Maruška, and L. Jonušauskas. Applied Physics A (2021). DOI: 10.1007/s00339-021-04872-4.
Femtosecond Laser-Based Integration of Nano-Membranes into Organ-on-a-Chip Systems
L. Bakhchova, L. Jonušauskas, D. Andrijec, M. Kurachkina, T. Baravykas, A. Eremin, and U. Steinmann. Materials 2020, 13, 3076 (2020). DOI: 10.3390/ma13143076.